The 97th New York Infantry Regiment ("Conkling Rifles") was an infantry regiment in the Union Army during the American Civil War.

The 97th New York Infantry was organized at Boonville, New York and mustered in for three years service on February 18, 1862 under the command of Colonel Charles Wheelock.

The regiment was attached to Wadsworth's Command, Military District of Washington, to May 1862.

1st Brigade, 2nd Division, III Corps, Army of Virginia, to September 1862.

1st Brigade, 2nd Division, I Corps, Army of the Potomac, to December 1862.

The 97th New York Infantry mustered out of service on July 18, 1865.

Picket duty on the Shenandoah River and at Front Royal to June 10.

Duty at Catlett's Station, Warrenton and Waterloo, Va., until August.

Pope's Campaign in northern Virginia August 16-September 2.

Pursuit of Lee to Manassas Gap, Va., July 5–24.

Duty on line of the Rappahannock and Rapidan until October.

Mine Explosion, Petersburg, July 30, 1864 (reserve).

Sussex Court House December 10, Dabney's Mills, Hatcher's Run, February 5–7, 1865.

Lewis Farm, near Gravelly Run, March 29.