It has been used to propel the upper stages of several launch vehicles, including the Delta II and Titan III.

The first AJ10-101 flight, with a Thor-Able, occurred on 23 April 1958; however, the Thor failed before the upper Able stage fired.

Trans-Earth injection, from lunar orbit, was the most critical usage of this engine during the Apollo program.

This version used Aerozine 50 (a 1:1 mix of UDMH and hydrazine) as fuel and nitrogen tetroxide (N2O4) as oxidizer, rather than the previous nitric acid/UDMH.

It was used by the Delta-F upper stage of the Delta 1000 Straight Eight series rocket, starting in 1972.