The other major portions of the reserve are in the Anuppur (16.20%) and Dindori (15.70%) districts of Madhya Pradesh.

The protected area of the Achanakmar Wildlife Sanctuary is located in the Bilaspur district within the Biosphere Reserve.

The area of the Achanakmar-Amarkantak Biosphere Reserve is considered to be one of the major watersheds of peninsular India.

[2] Maikal hill ranges together with Vindhya and Satpura lie within the Achanakmar-Amarkantak Biosphere Reserve.

The typical monsoon climate of the reserve has three distinct seasons: Summer (March–June), Rainy (July–October), and Winter (November–February).

The reserve is quite rich in plant diversity, having a combination of different climatic and edaphic conditions at various altitudes.

Some of the important species include Thalictrum sp., Dillenia pentagyna, Cocculus hirsutus, Flacourtia indica, Talinium portulacifolium, Tamarix ericoides, Abelmoschus ficulneus, Hibiscus subdariffa, Corchorus fascicularis, Grewia rothii, Biophytum sp., Oxalis sp., Tropaeolum majus, Gloriosa superba, Curcuma aromatica, Dioscore sp., Chlorophytum tuberosum, Curculigo orchioides, and Hypercum japonicum.