In histology, it is used as a fluorescent stain, and as a fluorescent probe for non-invasive measurements of cytoplasmic pH changes in whole cells.
Acridine yellow damages DNA and is used as a mutagen in microbiology.
According to a publication by Karl Drechsler, a student of Guido Goldschmiedt at the Imperial and Royal University of Vienna, Moriz Freund discovered the substance in 1896 during experiments at the University of Prague.
Drechsler was then able to produce the substance in larger quantities and subsequently also examine it more closely.
This article about an organic compound is a stub.