[1] The Humana Festival has introduced nearly 450 plays into the American and international theatre's general repertoire, including three Pulitzer Prize winners—The Gin Game by D. L. Coburn, Crimes of the Heart by Beth Henley and Dinner with Friends by Donald Margulies—as well as Marsha Norman's Getting Out, John Pielmeier's Agnes of God, Charles Mee's Big Love, Naomi Iizuka's Polaroid Stories and At the Vanishing Point, Jane Martin's Anton in Show Business, Rinne Groff's The Ruby Sunrise, Theresa Rebeck's The Scene, Gina Gionfriddo's After Ashley and Becky Shaw, UNIVERSES' Ameriville, Rude Mechs' The Method Gun, Dan O'Brien's The Cherry Sisters Revisited, Jordan Harrison's Maple and Vine, Will Eno's Gnit, Branden Jacobs-Jenkins' Appropriate, and Lucas Hnath's Death Tax and The Christians.

Former executive director, Alexander Speer, whose tenure of forty years began in 1965, became Jory's partner and led the theater's administration and operations until his retirement in the spring of 2006.

During his tenure at Actors Theatre, Masterson produced more than 200 plays and expanded and established an Education Department consisting of public outreach programs including classroom workshops, artists in the schools, increased weekday student matinées, backstage tours and professional development for teachers and community center leaders.

[12] The original home of Actors Theatre was an open loft—the former Egyptian Tea Room—above the Taylor Trunk Company on Fourth Street in downtown Louisville.

In 1965, the theater relocated to the former site of the Illinois Central Railway Station on Seventh Street and River Road.

In October 1972, the theater relocated to the newly renovated Old Bank of Louisville building on Main Street, where it remains to this day.