On 20 December 2016, the Boeing 727-2J0F operating the route overran the runway during takeoff, striking the perimeter fence and other obstacles before becoming airborne, ultimately losing control and crashing 4 nmi (4.6 mi; 7.4 km) from the airport.

[3] The Boeing 727 arrived at Puerto Carreño on the day of the accident at 14:48 local time, after a cargo flight from Bogotá.

Although the weight and balance manifest of the accident flight was not found, investigators presumed that slightly less than 9,100 kg (20,000 lb) of cargo distributed on nine pallets was loaded for the return to Bogotá.

It then crossed a road on the airport's perimeter, where numerous motorcyclists and pedestrians narrowly avoided being hit by the aircraft.

The aircraft achieved an altitude of 790 ft (240 m), entered a slight right-hand descending turn, which completed a near 270° arc, and then impacted flat terrain, bursting into flames.

[10] The Colombian government's Air Accident Investigation Group (Spanish: Grupo de Investigación de Accidentes Aéreos; GRIAA) concluded that three factors extended the aircraft's takeoff run by 380 m (1,250 ft), resulting in a runway excursion that eventually led to the crash: Furthermore, although initial calculations suggested the aircraft was operating within its weight limits, the investigators believe, based on the takeoff speeds used by the crew, that the aircraft was actually almost a tonne above its maximum permissible takeoff weight of 74.7 t (74,700 kg; 165,000 lb).