Affinity capture

Affinity capture is a technique in molecular biology used to isolate desired compounds based on their chemical properties and a solid substrate.

[1] Commonly, plates out of solid materials such as glass are coated with various reagents to allow for covalent bonding of a capturing molecule such as an antibody.

Washing the plate and removing the desired compound completes the purification process.

[2] Affinity capture has been used to isolate proteins by means of binding a peptide sequence to the solid substrate, thus allowing for protein capture.

[3] The process has also been examined for potential automation, but the unique circumstances for any given experiment may impede reproducibility.