[1] The exhibition was made up of artworks and historical artifacts from and about the African diaspora, specifically focusing "on the 'ebbs and flows' among Africa, Americas, Caribbean and also Europe.

"[1][2] Built around the concept of histórias, a Portuguese term that can include fictional and non-fictional narratives, Afro-Atlantic Histories explores the artistic, political, social, and personal impacts and legacies of the Transatlantic slave trade.

[1] Artists in the original exhibition included Maxwell Alexandre, José Alves de Olinda, Sidney Amaral, Benny Andrews, Emanoel Araújo, Thomas Jones Barker, Agostinho Batista de Freitas, John T. Biggers, Skunder Boghossian, Luiz Braga, Agostinho Brunias, Flávio Cerqueira, Timótheo da Costa, Mário Cravo, Jr., Jean-Baptiste Debret, Beauford Delaney, Mestre Didi, Aaron Douglas, David Driskell, Augustus Earle, Albert Eckhout, Melvin Edwards, Ibrahim El-Salahi, Ben Enwonwu, Nona Faustine, Théodore Géricault, Adenor Gondim, Titus Kaphar, Victor Patricio Landaluze, Jaime Lauriano, Ernest Mancoba, Edna Manley, David Miller Sr., Marepe, Paulo Nazareth, Uche Okeke, Moisés Patrício, Dalton Paula, Rosana Paulino, Frans Post, Edouard Antoine Renard, Faith Ringgold, Glauber Rocha, Vincent Rosenblatt, Cameron Rowland, Pascale Marthine Tayou, Hank Willis Thomas, Rubem Valentim, Carlos Vegara, Julien Vallou de Villeneuve, Barrington Watson, Osmond Watson, Ellis Wilson, and Lynette Yiadom-Boakye.

[3][2] Artists with works new to the exhibition on the American tour include Ernest Crichlow, Theaster Gates, David Hammons, Arthur Jafa, Glenn Ligon, Daniel Lind-Ramos, Zanele Muholi, Faith Ringgold, Alma Thomas, Kara Walker, and John Quincy Adams Ward.

[52] Following the opening of the American tour at the NGA, Philip Kennicott wrote in The Washington Post that the mixture of artistic media & time periods in the show "makes for some stunning juxtapositions," adding that the show "has tremendous symbolic importance for the National Gallery" due to its location in the NGA's West Building, the wing of the museum traditionally devoted to canonical artworks that has long included very few Black artists.

[4] Describing the NGA exhibition in ARTnews, Alex Greenberger wrote that "though Afro-Atlantic Histories features depictions of violence, it also proposes that, under the most dehumanizing circumstances, Black people across the world found means of self-possession.

Lucas also praised the juxtapositions of works in the show; specifically, the contrasts between Arthur Jafa's Ex-Slave Gordon (2017), Eustáquio Neves' Untitled (1995), and McPherson & Oliver's The Scourged Back (c.1863); between Sidney Amaral's Neck Leash (Who Shall Speak on Our Behalf?)

Writing about the "Portraits" section of the exhibition, Lucas noted that works like Flávio Cerqueira's Amnesia (2015) and Samuel Fosso's Self-Portrait (as Liberated American Woman of the ’70s) (1997) represent "contemporary challenges to the erasure of Blackness in the West.