[1] Often partnered with the junoesque Olga Lawina (an agent of the Swiss Secret Service), his adventures take him around Europe and the rest of the world as he battles numerous villains, both fictional and parodies of real people.

The song "Denk toch altijd met liefd' aan je moeder" by Gerda en Herman Timmerhout is a common feature in the comic, and is often used to hideously torture someone or provide a plot-turning emotional reaction.

Agent 327 debuted in 1966 as a feature in Pep magazine #21, written and drawn by Martin Lodewijk and published by Geïllustreerde Pers.

On May 15, 2017, the Blender Foundation[3] released a teaser trailer that was almost four minutes long,[4] for an upcoming animated feature film based on the comic.

Roosendaal revealed the working title of the full feature film as: Agent 327, The Case of the Einstein Enigma.