The Alabama, Florida and Gulf Railroad was an American railroad which ran from Ardilla, Alabama to Greenwood, Florida.
The line started as a logging railroad owned by the E.L. Marbury Lumber Company of Dothan, Alabama, running from Ardilla to Malone, Florida, where the company had a sawmill.
The railroad was placed in receivership in 1924 and sold after foreclosure to the Bank of Dothan.
The primary motive power left on the railroad was a diesel railbus.
The railroad ceased operation in 1941 and the equipment was sold for scrap.