Krotoski was born a U.S. citizen in Kuala Lumpur, Malaysia, but spent her early years in New Orleans, Louisiana.

[9] Her parents, Wojciech Antoni "Al" Krotoski (1937–2016) and his then-wife Danuta (née Gwozdziowski), were Polish-American scientists who played a key role in revealing hypnozoites as the true mechanism of malarial relapse.

"[14] From 1999 to 2001 she co-presented Channel 4's late evening video gaming review show, Bits with Emily Booth and Emily Newton Dunn,[15] and from 2001 to 2002 co-presented video game review programme Thumb Bandits alongside Iain Lee.

[16] In 2006, she contributed to the United Kingdom's Department for Education and Skills and the Entertainment and Leisure Software Publishers Association (ELSPA) collaboration, "Unlimited Learning: The role of computer and video games in curriculum-based education".

This TV documentary series was described by the BBC as charting "two decades of profound change since the invention of the World Wide Web, weighing up the huge benefits and the unforeseen downsides.