His parents, Lyudmila Georgievna and Alexander Mikhailovich Misurkin, reside in Oryol, Russia.

He continued pilot training at the Armavir Military Aviation Institute, and graduated in October 1999 with a gold medal as a pilot-engineer.

From August 2009 to February 2011 he took advanced training specializing in the International Space Station (ISS) program.

In 2014, he participated in the ESA CAVES[3] mission of the European Space Agency alongside Scott Tingle, Luca Parmitano, Sergey Kud-Sverchkov and Matthias Maurer.

On 2 February 2018, Misurkin along with flight engineer Anton Shkaplerov participated in an 8-hour 13 minutes spacewalk outside of the ISS to replace an old electronics box for a high-gain communications antenna.