He then entered the Kacha High Air Force Pilot School, where he studied to September 1998.

He continued pilot training at the Armavir Military Aviation Institute, and graduated in October 1999 with a gold medal as a pilot-engineer.

From August 2009 to February 2011 he took advanced training specializing in the International Space Station (ISS) program.

In 2014, he participated in the ESA CAVES[3] mission of the European Space Agency alongside Scott Tingle, Luca Parmitano, Sergey Kud-Sverchkov and Matthias Maurer.

On 2 February 2018, Misurkin along with flight engineer Anton Shkaplerov participated in an 8-hour 13 minutes spacewalk outside of the ISS to replace an old electronics box for a high-gain communications antenna.