Alfonso de la Mota y Escobar was born in México on 18 May 1546.
[3][4] On 31 March 1594, he was appointed during the papacy of Pope Clement VIII as Bishop of Nicaragua but resigned in 1595.
[3] On 11 March 1598, he was appointed during the papacy of Pope Clement VIII as Bishop of Guadalajara.
[2][3] In June 1599, he was consecrated bishop[3] On 11 March 1598, he was appointed during the papacy of Pope Clement VIII as Bishop of Tlaxcala (Puebla de los Angeles).
[1][3] He served as Bishop of Tlaxcala (Puebla de los Angeles) until his death on 16 March 1625.