Before Akbar, he had also served under Humayun and later commanded the Mughal army to the eastern Uttar Pradesh region in battle against the rebel Afghans.

Ali Quli Khan had kinship ties with other leading Uzbeg nobles of the Akbar who enjoyed powerful position and prestige during the time of Humayun as well.

[1] Akbar was also skeptical of the Khan Zaman's conduct, who fell in love with the son of a Camel rider whom he called "My Padshah" and bowed down to him.

Akbar also commanded his nobles to build houses in the Jaunpur to undertake a long run campaign against the rebels until they are crushed.

The attempt of the Uzbegs under Ali Quli Khan to create dissatisfaction in the empire resulted in loss of Akbar's trust upon them.