His last communiqué to the CIA contained highly sensitive intelligence regarding Sydney's nemesis, Anna Espinosa, formerly a top agent for K-Directorate, now gone rogue.

She is assisted by her back-up team: Marcus Dixon, her field partner and loyal friend; Michael Vaughn, her CIA handler; and Marshall Flinkman, a socially inept technological whiz.

Jacobs' data leads the team to a museum in Saudi Arabia proudly displaying a mysterious set of bones found in the desert with links to Rambaldi.

While Sydney was in the desert, Vaughn has tracked both missing agent Jacobs and a mathematician, Dr. Caplan, to an insane asylum in Romania.

Breaking into the asylum, Sydney discovers that the prisoners are being used as human guinea pigs for Anna and Sark's experiments with their laser.

Trading his intelligence for immunity, Sark tips the team off that Arvin Sloane is cutting a specific diamond for Anna in a laboratory beneath an embassy in Hong Kong.

As Anna marches off a double-crossed Sloane, the CIA operatives receive a surprise visit by the Followers of Rambaldi SWAT team.

Reluctantly agreeing to an uneasy truce with him, Sydney must defeat Anna and figure out how to destroy the mighty Machine to stop ecological disaster being launched on the whole planet.