[2] When originally created in 2009, Alicyclobacillaceae was a monophyletic family, only including genus Alicyclobacillus.

Certain Alicyclobacillus species (especially A. acidoterrestris) have been implicated in spoilage of pasteurized fruit juice.

Both species of Kyrpidia have been isolated from areas of high volcanic activity in Tuscany, Italy, and the Azores.

Members of this genus have been isolated from arctic permafrost, soil samples, cassava wastewater, decomposing algal scum, river water, and the gut of a vulture.

[9] Tumebacillus was found during surveys of nasal airways of infants, an underground subway in Norway, and a mountain observatory in Austria.