Allomyces macrogynus

[4] The genome of Allomyces macrogynus has been sequenced[2] and this makes it desirable to review an organism of interesting structure and one which is responsive to environmental changes in easily observable ways.

The vegetative growth showed the formation of rhizoids, hyphae and branching and then in the diploid cultures two kinds of fruiting body, zoosporangia ZS that reproduced the diploid organisms and resting or resistant sporangia RS that led to the haploid organism.

[10] The diploid organisms can produce zoosporangia ZS when conditions are good and the resistant or resting sporangia RS when they are unfavourable.

Zoospores encysted and attached to the unshaken glass vessel and the CH could then be removed and replaced with defined medium.

[15] For chemically defined induction of germination mixtures of leucine and lysine or phenylpyruvate were the best of many compounds tested.

The synchronously growing hyphae showed development at the hyphal tip in G1 of the growth cycle and widening at the base in G2.

A further departure from apical growth was observed if hyphal organisms, growing on the surface of solid media, were covered with a microscope slide to create an oxygen gradient.

[9] The latter study also showed effects of growth in applied voltages and chemotropism of the rhizoids to casein hydrolysate.

Indeed, it is still not clear how the error occurred because stability constants for EGTA chelated with Fe, Zn and Mn had been published[20] before any claim of specificity for Ca.