Alpha Epsilon Iota (ΑΕΙ) was an American professional fraternity for women in the field of medicine.

Alpha Epsilon Iota was established as a medical fraternity for women on February 26, 1890 at the University of Michigan.

Its Founders were: Founded prior to the turn of the 20th Century, Alpha Epsilon Iota was thereby one of the earliest of the professional medical fraternities to serve women.

Minnesota's Epsilon chapter remained active through the 1970s at which time there was a marked expansion of female enrollment in the growing medical school there, but ΑΕΙ sold its properties in 1979, splitting then into two groups, the AEI Foundation which continues to provide scholarships, and a networking organization called Minnesota Women Physicians.

The official badge is described as a "black enameled faceted pentagon, having a gold star at the apex with an emerald inset.