Her father, Antonín Mánes, brothers Josef and Quido, and uncle Václav were all painters.
Although she wanted to specialize in portraits, her father believed it was inappropriate for a woman to do so and insisted that she do landscapes instead.
[1] When Mánes became ill in 1866, Mánesová took him to Rome for his health, then back to Prague, where she cared for him until his death in 1871.
During this time, she turned down an offer of marriage from Václav Levý.
[1][2] Mánesová died suddenly from heart disease.