In 1994, he broke the school's single-season record with 1,096 receiving yards and was a finalist for the Biletnikoff Award and a first-team All-Big Ten honoree.

Toomer finished his college career ranked second (now fourth) in Michigan history with 2,657 receiving yards.

[4] Toomer began playing Pee-Wee Football as a lineman for the Berkeley Cougars and as a receiver, kicker, punter, linebacker, and running back for the Richmond Steelers.

[6] As a freshman and sophomore in 1992 and 1993, he started only three games (two in 1992 and one in 1993) and was principally used as a backup to the team's #1 receiver, Derrick Alexander.

[10] Despite technically being a "backup" to Hayes, Toomer in 1994 broke Jack Clancy's single-season Michigan record with 1,096 receiving yards.

[14] Toomer was a finalist for the Biletnikoff Award in 1994 and was selected by both the conference coaches and media as a first-team wide receiver on the 1994 All-Big Ten team.

[12] As a senior, Toomer finally became a starter, starting 12 games at split end for the 1995 Michigan team.

[15] However, Toomer was routinely double-teamed in 1995,[16] opening the way for Mercury Hayes to lead the team in both receptions and receiving yardage.

[25] Toomer later publicly acknowledged that his slow start as an NFL receiver was the result of exercise-induced asthma which he had hidden for years.

In 2000, Toomer appeared in all 16 regular season games (14 as a starter) for the Giants and posted solid totals with 78 receptions for 1,094 yards and seven touchdowns.

In a Week 2 game against the Philadelphia Eagles, he tallied a career-high 12 receptions and scored two touchdowns to help spark a 17-point comeback.

[28] Toomer's season came to an end after eight games when he suffered a partially torn ACL in his left knee.

Giants head coach Tom Coughlin noted, "There's no question Amani has a very positive effect on Eli.

On October 21, 2007, in a home game against the San Francisco 49ers, he broke Kyle Rote's club record with his 49th career touchdown catch.

[33] The 2007 Giants advanced through the playoffs and won Super Bowl XLII over the previously undefeated New England Patriots.

His first touchdown gave the Giants a 7–0 lead after he caught an 11-yard pass, eluded three would be tacklers, and ran 50 yards for the score.

In May 2008, Toomer and the Giants were invited by U.S President George W. Bush to the White House to honor their victory in Super Bowl XLII.

[34][28] In 2010, Toomer was included in the initial group selected for induction into the New York Giants Ring of Honor.

[37] In 2011, Toomer and fellow former New York Giant Roman Oben joined MSG Varsity's coverage of "Friday Night Football.