Ambrose J. Murphy (1869–1949) was an American architect whose practice was based in Providence Rhode Island.

He was a specialist in ecclesiastical work and, in a career that spanned over 40 years, designed many buildings for the Roman Catholic Diocese of Providence Rhode Island and Fall River Massachusetts.

He began his architectural career with his uncle James Murphy (1834–1907), an ecclesiastical architect in Providence, Rhode Island.

Ambrose Murphy began his long and distinguished career at roughly the same time that Charles Maginnis of Boston, Massachusetts, was beginning his.

His unique ecclesiastical buildings are said to be much admired locally and have found their way to some of the National Registers.