Ambrose University is the product of an educational journeys which began in the second half of the twentieth century.

It was established under Gordon Skitch, superintendent of the Western Canadian District of the CMA, Willis Brooks, pastor of the Regina Alliance Tabernacle and George Blackett, former principal of Winnipeg Bible Institute and future principal and president of the new institute.

In 1960, CNC moved to Winnipeg and was established as the official Canadian university college for the Church of the Nazarene.

In 2003, CBC/CTS officially relocated to join NUC on the same campus, received accreditation in 2004, and changed its name to Alliance University College (AUC).

[5] In 2014, Premier and Minister of Innovation and Advanced Education Dave Hancock approved the change of name to Ambrose University, dropping the word "College" to better reflect the nature of the institution.

[6] In April of 2023, Dr. Smith announced a plan to retire from his role as president and began the process of transitioning out of leadership.

[7] At that time, the Board of Governors Presidential Search Committee began its process of finding a candidate for the presidency.

[13] Ambrose's mission is to "produce graduates who are consciously and actively Christian in all aspects of life," and to "serve the church by providing excellent preparation for pastoral ministry.".

Ambrose is accredited by the Association of Theological Schools in the United States and Canada to offer its Seminary degrees.