Eggs are laid on leaf litter and the adult transports tadpoles to wet trenches or small rocky pools.

[1] Lutz's poison frog males call for females in breeding sites, such as rock crevices, termite nests, low vegetation[6] or trenches.

[7] Breeding periods for this species were seen to begin with male calling during months of October to December, then sights of tadpoles were during November to April, and finally froglets were recorded during January to April.

It does face some localized threat from habitat loss associated with farming, mining, logging, and livestock cultivation.

While there is both breeding and wild capture for the international pet trade, scientists do not believe this poses a threat to the population.