She was born in June 1955, to the late Serwano K. Kulubya and Mary Kafureka, in Guildford, Surrey, United Kingdom.

Amelia Kyambadde holds the degree of Bachelor of Business Administration (BBA), from Makerere University, Uganda's oldest institution of tertiary education, founded in 1922.

She also holds the degree of Master of Business Administration (MBA), from the American InterContinental University in London, United Kingdom.

[10] In 2010, she voluntarily resigned as the President's PPS, to contest the Parliamentary seat of Mawokota County North, Mpigi District.

She is the Patron of Twezimbe Development Foundation (TDF), a community-based, non-governmental, organization formed to improve the living conditions of the people of her parliamentary constituency.