The American 1000 Guineas was an American Thoroughbred horse race held annually at Arlington Park in Arlington Heights, Illinois.

Inaugurated in 2008, the race was open to three-year-old fillies and was contested on turf over a distance of one mile (eight furlongs).

The race was dropped from Arlington Park's stakes schedule for 2011.

Speed record: Most wins by an owner: Most wins by a jockey: Most wins by a trainer: