The American Samoa delegation included six athletes: sprinter Nathan Crumpton, sailors Adrian Hoesch and Tyler Paige, swimmers Micah Masei and Tilali Scanlan, and weightlifter Tanumafili Jungblut.

[11] The nation selected Nathan Crumpton,[12] a skeleton athlete who previously represented the United States in international competition.

[14] Before the Games, Crumpton had never competed in any sprinting event, as his only athletics experience beforehand was being a long and triple jumper for Princeton University.

[16][17] American Samoan sailors qualified one boat in each of the following classes through the class-associated World Championships and the continental regattas, marking the country's recurrence to the sport for the first time since the 1996 Summer Olympics in Atlanta, Georgia.

[18] The pair, Adrian Hoesch and Tyler Paige, qualified by winning the 2020 Sail Melbourne International for the Men's 470 class spot.

[25] Masei competed in his event on July 24, 2021, in the first heat against Muhammad Isa Ahmad of Brunei and Amini Fonua of Tonga.

[27] The following day, Scanlan competed in her event in the second heat against Andrea Podmanikova of Slovakia, Phee Jinq En of Malaysia, Ema Rajić of Croatia, Emily Santos of Panama, Kirsten Fisher-Marsters of the Cook Islands, Alicia Kok Shun of Mauritius, and Claudia Verdino of Monaco.

[29] American Samoa received one tripartite invitation quota from the International Weightlifting Federation, picking Tanumafili Jungblut, who competed in the men's 109 kg category.

[30] Jungblut was a returning Olympian, first competing in the men's 94 kg category at the 2016 Summer Olympics in Rio de Janeiro, Brazil,[31] placing without a rank after failing to complete three snatches.