Amfonelic acid (AFA; WIN 25,978) is a research chemical and dopaminergic stimulant with antibiotic properties.

[1] In addition to behaving as an antibiotic, it was found that many derivatives of nalidixic acid have either stimulant or depressant effects on the central nervous system.

[2] Researchers at Sterling-Winthrop found that AFA had a higher potency and therapeutic index than cocaine or amphetamine, leading to further study of the drug.

[1] In studies, it proved to be a potent and highly selective dopamine reuptake inhibitor (DRI) in rat brain preparations.

[6] Despite lack of direct serotonin activity, rats treated with subchronic doses of amfonelic acid display subsequent decreases in 5HT and 5HIAA.