Ami Assaf (Hebrew: עַמִּי אָסָף; 22 July 1903 – 17 May 1963) was an Israeli politician who served as a member of the Knesset for Mapai from 1949 until 1961.

Born Ami Vilkomitz in Rosh Pinna, Assaf attended the Herzliya Gymnasium.

One of the founders of the Kfar Yehoshua moshav, in 1936 he became secretary of the Moshavim Movement, and was a member of Mapai's central committee.

On 28 December 1959 he was appointed Deputy Minister of Education and Culture.

After re-election in 1961 he reprised the role, holding it until his death in 1963.