He traveled to Rome with his father in 1496, and is briefly documented there again between 1500 and 1503, returning to Bologna thereafter and painting in a style influenced by Pinturicchio and Filippino Lippi (whose work the critic Roberto Longhi suggested [in Officina ferrarese, 1934] he may have seen in Florence before 1500).
In Bologna in 1504, he joined Francia and Costa in painting frescoes for the Oratory of Santa Cecilia next to San Giacomo Maggiore, a work commissioned by Giovanni II Bentivoglio.
In 1508–1509, while in exile from Bologna following the fall of the Bentivoglio family, Aspertini painted the splendid frescoes in the Chapel of the Cross in the Basilica di San Frediano in Lucca (a church, like the Oratory of Santa Cecilia, maintained by Augustinian friars).
Aspertini was also one of two artists chosen to decorate a triumphal arch for the entry into Bologna of Pope Clement VII and Emperor Charles V in 1529.
For example, the Pietà he painted inside San Petronio appears to occur in an other-worldly electric sky.