He served as part of a force securing traffic arteries in the Gaza Strip and as a road accident investigator in southern Israel, completed an officer's course and was a commander of the Karni crossing.

Ohana eventually entered the Knesset on 27 December 2015 as a replacement for Silvan Shalom after he resigned amidst a sexual harassment scandal.

[10] After being re-elected in the April 2019 elections he was appointed Minister of Justice in June, becoming the first openly LGBT individual to serve in the government.

His appointment came after controversial comments from Bezalel Smotrich, another contender for the office, saying that he would like to establish a halachic state governed by Jewish religious law.

[17] Ohana is a supporter of loosening the policy on carrying firearms in Israel in order to allow more armed people on streets for prevention and fast reaction to terror attacks.

[19] When opposition members of Knesset in February 2016 proposed several bills intended to improve the gay community's status such as recognizing bereaved widowers, banning conversion therapy, recognizing same-sex marriage contracts and to train health professionals to deal with gender and sexual orientation issues, Ohana left the session without voting; he said he could not vote against these bills, but he did not want to violate the party line.

[20] In 2018, he authored and voted for an amendment to Israel's surrogacy law to extend rights to same-sex couples and men.

[8] Ohana and his husband Alon Hadad have a son and a daughter, both born through a surrogate mother in the US state of Oregon.