Her best-known role was as Lupita, the mother of the Vera household in the Ecuadorian sitcom Mis adorables entenados [es].

[1][2] She took an interest in singing and acting from a young age, and at 23, began her career in the field by joining a theater group.

The next year, Ecuavisa brought the production to television and Guillén played the mother of the eponymous Vera family, who struggled to raise her step-children.

[4] Amparo Guillén was sexually assaulted at ages six and fourteen, causing her to fall into substance abuse for the next 21 years of her life.

She sought help, and at age 41, defeated her addiction and spoke publicly to young people about the dangers of substance abuse.