[2] The club was founded in 1970 [3] in the Ampelokipoi (formerly Tampakika) district of Larissa, on the banks of the Pineios river that crosses the town.

Since their relegation in 1994, Ampelokipoi never managed to play in a national division but they have been a dominant force in the Larissa FCA leagues, especially during the late 1990s.

On 25 August 2011, Ampelokipoi's chairman Andreas Mpalanikas signed a 5-year plan agreement with the Ajax Youth Academy in Larissa for a future co-operation and development of the young talents of the club.

The ground that has a capacity of 300 has hosted Delta Ethniki matches from 1991 until 1994 and is considered one of the most historic and iconic in the town of Larissa.

[11] Kostas Paparizos, the team's former coach is another footballer who started his career from Ampelokipoi Larissa and remained until the age of 18, when he was transferred to AEL in 1999.