The club runs six senior teams and the full range of junior sides.

[2] The first XV play in the RFU Championship, the second tier of the English rugby union system.

In 2013–14, the team finished second but lost the promotion play-off to Darlington Mowden Park of the National League 2 North.

They beat Bishop's Stortford, the National League 2 South runner-up in the promotion play-off at Dillingham Park, 19–10.

[8] The Ampthill squad for the 2023–24 season is:[10][a][b] Note: Flags indicate national union under World Rugby eligibility rules.