Changing exhibits featuring photography, ephemera, and artifacts from the museum's collection, add depth and incidental information to the overall story of railroad history.
The museum has an extensive educational program for elementary students from across the region to help them learn about railroad history using re-enactments, costumed docents, and including train and handcar rides.
When not on display, these items are stored and worked on at the nearby Sacramento Railyards in the remaining buildings that were part of the original Southern Pacific Shop complex.
In early 2011, the interior remained closed to public use, but is occasionally open for special events.
Between April and October, the Sacramento Southern Railroad, operated by the museum, takes passengers on a 40-minute, 6-mile (9.7 km) roundtrip route along the Sacramento River on a portion of the Walnut Grove branch of the former Southern Pacific Railroad.