In his will, Eugène provided equally for his children, but the portions for Amy and her sisters were held in trust for them, while their brothers received their inheritance outright.

[3] She served on the University of Delaware Board of Trustees' Advisory Committee on the Women's College from 1939–1944.

In 1939, she and her attorney, Judge Hugh M. Morris, founded the Unidel Foundation, a private charitable organization whose mission is "to aid and promote higher education in the State of Delaware, and to increase, enlarge and improve the scientific and educational advantages and opportunities of its people by gifts and contributions [to the university].

"[3] Without any children, Amy willed much of her inheritance to the Unidel Foundation, including DuPont and Hercules Inc. stock valued at some $25 million at her death in 1962.

[6][7] The first major project was to renovate the Carpenter Sports Building, named in honor of du Pont's cousin R. R. M. Carpenter (1877–1949), a DuPont executive, owner of the Philadelphia Phillies and long-time benefactor for the university's athletic department.