Born in Redmond, Washington, Tryon earned a bronze medal in team eventing at the 2004 Summer Olympics in Athens.

She competed alongside Kimberly Severson, John Williams, Darren Chiacchia, and Julie Richards.

At the Rolex Kentucky cross-country on April 28, 2007, Amy Tryon continued to ride for approximately 30 seconds after her horse, Le Samurai, had sustained what later proved to be a fatal injury.

Despite the horse being visibly lame, Tryon chose to jump the final fence, which worsened the injury, making it irreparable.

Toxicology reports revealed toxic levels of oxycodone, diphenhydramine, alprazolam, lorazepam, diazepam, and temazepam in her system at the time of her death.