She later jumped ship to Telecinco to host El programa de Ana Rosa, which led the ratings continually from 2005 until it was axed in 2023.

In 2005, Quintana interviewed Ramona Maneiro, who admitted on Sabor a ti (es) to having assisted Ramón Sampedro in taking his own life.

In September of that year it was announced that she would return to Telecinco to launch a new show competing with María Teresa Campos, whose programme Cada día replaced Sabor a ti on Antena 3.

[8] Launched on 18 January 2005, El programa de Ana Rosa quickly rose to lead the timeslot on Telecinco, with Cada día being axed after just one year.

"[11] After failing to meet expectations, going up against Sonsoles Ónega on Antena 3, Telecinco announced that El programa de Ana Rosa would return at the start of February 2025.

It was initially blamed on a computer fault, yet once a corrected version went on sales the Interviú magazine noticed other passages plagiarised from Ángeles Mastretta's 1985 short story Women with Big Eyes.

[13][14][15][16][17] On 25 February 2011, the wife of murderer Santiago del Valle was sent to prison after admitting on El programa de Ana Rosa that she knew he had in fact killed five-year-old girl Mari Luz.

Some days later, the newspaper El Mundo criticised Ana Rosa's treatment of Ms. del Valle in getting the scoop, and the police opened an investigation into her and Telecinco.

[19] In April 2011, the Supreme Court ordered Ana Rosa and Cuarzo Producciones to pay bullfighter Finito de Córdoba and his wife, former TV presenter Arantxa del Sol, €100,000 for defamation, after commenting on the show about rumours of his infidelity.

[22] In 2017, after denouncing violence against women on a TV special about her, La reina de las mañanas, she was recorded remarking that "it's good to me that she did his face in", not realising her microphone was still on.

After social media users criticised a perceived double standard on these comments, Ana Rosa alleged her words had been taken out of context.

[23] On some occasions, Ana Rosa has been accused of giving misleading information in favour of her personal political, economical or social interests.