Ana was born to her father was Francisco Villamil y Garaicoa, and mother Isabel María Icaiza y Paredes.

[citation needed]Her father was the son of José de Villamil, an Ecuadorian Independence leader.

Her mother Isabel was the daughter of Francisco de Paula Icaza and Isabel Paredes y Olmedo; niece of the poet Magdalena, and sister of José Joaquin de Olmedo.

[1] Ana worked as music teacher at municipal schools in her hometown throughout her life.

[2] She lived in Guayaquil at "10 de Agosto and Chimborazo," which is now considered to be a historic site.