Analytical profile index

The analytical profile index, or API, is a classification system for bacteria based on biochemical tests.

[3] The API range introduced a standardized and miniaturized version of existing techniques, which were considered complicated to perform and difficult to read.

During incubation, metabolism produces color changes that are either spontaneous or revealed by the addition of reagents.

[5] If the culture is determined to be oxidase-positive, alternative tests must be carried out to correctly identify the bacterial species.

This system is particularly useful in clinical microbiology and environmental studies for identifying bacteria based on their biochemical activities.