He is a Clyde and Helen Wu professor of Chemical and Systems Biology at Columbia University Irving Medical Center.

He also has roles in the Departments of Biochemistry & Molecular Biophysics, Biomedical Informatics, and Medicine.

[6] Califano was appointed president of the Chan Zuckerberg Biohub New York in 2023.

[7] Califano is renowned for his work in reverse engineering gene regulatory networks and analyzing them to identify key tumor checkpoint modules.

[8] This research has led to several clinical trials, including an N-of-1 study that analyzes patients with 14 untreatable, lethal cancers individually to prioritize therapeutic strategies.