Although the Wars of Independence, in which Scotland twice resisted attempted conquest by Plantagenet kings of England, formally ended in the treaties of 1328 and 1357 respectively, relations between the two countries remained uneasy.
Incursions by English kings into Scotland continued under Richard II and Henry IV and informal cross-border conflict remained endemic.
Formal flashpoints on the border included places remaining under English occupation, such as Roxburgh Castle and the port of Berwick-upon-Tweed.
This broke down after the accession of the more overtly bellicose Henry VIII to the English throne and James IV's catastrophically misjudged incursion into Northumbria in 1513 ending in the Battle of Flodden.
Three decades later, after the death of James V in 1542, the so-called 'rough wooing' at the hands of invading English armies under the Earl of Hertford brought manifest depredations to Scotland.
These battles were the result of England's ongoing military campaigning in France and Scottish attempts to support the House of Valois.
Tension between England and Scotland increased once again; not least because Henry had already broken with the Roman Catholic Church and embarked upon the Dissolution of the Monasteries, whereas James held to Rome and gave authority to powerful prelates such as Cardinal David Beaton.
Once again there were preliminary border skirmishes, but when James sent a large army into England, its leadership was weak and divided and it suffered a humbling defeat at the Battle of Solway Moss.
Campaigning continued the next year, but some Scottish factions reconciled and won a victory at the Battle of Ancrum Moor, which temporarily halted English attacks.
Elizabeth was able to ensure victory for the Protestant faction by using her fleet to blockade the Catholics and prevent French aid reaching them.
[6] For the later part of the 16th century, peace was ensured by the probability that James VI of Scotland, who was raised as a Protestant and was the son of Mary, Queen of Scots, would become King of England on the death of Elizabeth.