[1] It is a soluble dye used as a biological dye,[2] in fluorescence microscopy, appearing a yellow-green colour after excitation with violet light.
[3] It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline.
[4] Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome,[5] as well as to reveal callose structures in plant tissues.
[6] It can also be used in other connective tissue stains, such as Mallory's stain,[5] Gömöri trichrome stain, and Carstair's Method.
[7] It is used in differential staining.