Adult Swim Brain Trust was released as a special feature on the Squidbillies Volume One DVD on October 16, 2007.

Adult Swim Brain Trust features Space Ghost of Space Ghost Coast to Coast and Cartoon Planet hosting a focus group discussion about the unofficial pilot of Squidbillies with Meatwad from Aqua Teen Hunger Force, Early Cuyler from Squidbillies, and Sharko from Sealab 2021.

The Cybernetic Ghost of Christmas Past from the Future from Aqua Teen Hunger Force makes a cameo as a caller.

After unsuccessfully attempting to perform the interview, Space Ghost is shot by Sharko, who is eventually killed by Early.

The special ends with the set in ruins and Early shooting the talking head of Space Ghost off of Meatwad, while a bear eats Sharko's dead body in the background.