The Ann Arbor Railroad (reporting mark AA) is a Class III Railroad that operates fifty miles of track from Toledo, Ohio, to Osmer, Michigan.

[5] The Ann Arbor Railroad operates 83 miles (134 km) of track between its main terminal, Ottawa Yard, in Toledo, Ohio, and Ann Arbor, Michigan, the interchange point with the Great Lakes Central Railroad and Norfolk Southern.

The Ann Arbor Railroad also leases and operates an industrial lead in Toledo owned by the Norfolk Southern.

A variety of different cargoes are transported ranging from grain to liquefied petroleum gas to wooden poles.

Hallett Tower, the only remaining operational interlocking tower in Ohio, ceased operation in September 2019 with all dispatching duties being shifted to the dispatch center in Pittsburg, Kansas.