[1] The first major contingent of Presbyterians to arrive in Queensland came as part of John Dunmore Lang's immigration scheme in the late 1840s.

The first Presbyterian services were conducted in a house at Kangaroo Point until 1851 when a small timber church was opened at South Brisbane.

For the benefit of North Brisbane residents, services were also conducted in the School of Arts building on the corner of Queen and Creek streets.

[1] During the mid 1960s, the future of the church was threatened when the Brisbane City Council sought to acquire the site as part of the redevelopment of King George Square.

Church and community pressure, however, ensured the survival of the building,[1] although the extension was subsumed in the construction of 145 Ann Street, King George Central.

The front elevation contains two portal doors inset within semi-circular arches with ogival mouldings and patterned decoration in the render, and a rose window above.

The ceiling comprises diagonal boarding with regularly spaced fretwork panels above exposed timber trusses.

[1] A two storeyed rendered masonry building which contains a hall and offices is attached to the church at the northern transept[1] (demolished).

Ann Street Presbyterian Church was listed on the Queensland Heritage Register on 21 October 1992 having satisfied the following criteria.

[1] The place has a special association with the life or work of a particular person, group or organisation of importance in Queensland's history.