Anna Maria was the eldest daughter of Landgrave William IV of Hesse-Kassel (1532–1592) from his marriage to Sabine (1549–1581), daughter of Duke Christopher of Württemberg.
She married on 8 June 1589 in Kassel Count Louis II of Nassau-Weilburg (1565–1627).
In 1590 the couple moved in with Louis's father, Albert in Ottweiler.
Anna Maria helped organize the poor relief and set up a court pharmacy.
Anna Maria was buried in the crypt of the collegiate church of St. Arnual in Saarbrücken.