Anna of Nassau-Dillenburg (21 September 1541 in Dillenburg – 12 February 1616 in Weilburg) was a countesses of the House of Nassau.
Anna was a daughter of Count William "the Rich" of Nassau-Dillenburg and his second wife, Countess Juliana of Stolberg.
She had the same name as her aunt Anna of Nassau-Siegen (1440/41–1514), who had died twenty seven years before she was born.
On that day in Dillenburg two other marriages in the House of Orange took place: Johann VI, Count of Nassau-Dillenburg married Elisabeth of Leuchtenberg, and Elisabeth of Nassau-Dillenburg married Conrad, Count of Solms-Braunfels.
In 1574 he inherited another part of Nassau-Saarbrücken after the death of Count John III.