Anne was the wife of George Gordon, 5th Earl of Huntly, Lord Chancellor of Scotland and a chief conspirator during the reign of Queen Mary.
James, who was declared legally insane on 9 April 1562, had aspired to marry Queen Mary,[2] and at one point in 1562, there was a rumour spread throughout the realm that he had planned to abduct her.
On her return in December, Anne was bought magnificent clothes to join Guise's household as a lady-in-waiting and maid of honour.
[10] The marriage produced four children: Anne's husband was warded at Dunbar Castle following his father's rebellion and death in August 1562 at the Battle of Corrichie.
Anne was well regarded by Mary, Queen of Scots, and she was the only Hamilton mentioned in her will made in June 1566 before the birth of Prince James.
George, shortly after Darnley's murder, persuaded Jean to divorce Bothwell enabling him to marry the widowed Queen Mary.
He witnessed the marriage contract between the queen and Bothwell, and he was part of the retinue that accompanied the couple on their return to Edinburgh just before their wedding.
Anne's brother Claud Hamilton led the vanguard of Queen Mary's troops at the Battle of Langside but the royalists were defeated by Regent Moray's forces.