Anne Killigrew (baptised 7 September 1607, died 6 July 1641) was a Lady in Waiting to Queen Henrietta Maria, and the first wife of George Kirke (d. 1675) who was Groom of the Chamber to Charles I of England.

The king attended their wedding, on 4 January 1627,[1] and gave the couple an 80-year lease on the royal manor of Sheriff Hutton, Yorkshire.

[5] She is known to have been painted twice by Anthony van Dyck, once standing by herself[1] and once seated with a fellow lady-in-waiting, variously suggested to be Anne Dalkeith[6] or Charlotte, Lady Strange, Later Countess of Derby.

[9] Thomas Wiseman wrote, "The Court is very sad by reason of a great mishap happened to a barge coming through London Bridge, wherein were diverse ladies, and amongst the rest Mrs Kirke drowned.

[12][13] It was reported that Queen Henrietta Maria, who had not been on the royal barge at the time of the accident, had "taken very heavily the news, and, they say, shed tears for her.